Anti-phospho-MAP4K1 (Ser171) antibody
| 英文名称 | phospho-MAP4K1 (Ser171) |
| 中文名称 | 磷酸化造血祖细胞激酶抗体 |
| 别 名 | MAP4K1(phospho S171); Hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; HPK1; Human Hematopoietic Progenitor Kinase; M4K1_HUMAN; Map4k1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen Activated Protein Kinase Kinase Kinase Kinase 1; Mitogen-activated protein kinase kinase kinase kinase 1. |
DATASHEET
Host:Rabbit
Target Protein:phospho-MAP4K1 (Ser171)
IR:Immunogen Range:RL(p-S)FI
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:11184
Swiss Prot:Q92918
Source:KLH conjugated Synthesised phosphopeptide derived from human MAP4K1 around the phosphorylation site of Ser171:RL(p-S)FI
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-MAP4K1(Ser171)) Polyclonal Antibody, Unconjugated (bs5494R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-MAP4K1 Antibody(bs-5494R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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