Anti-phospho-MEF2D (Ser444) antibody
| 英文名称 | phospho-MEF2D (Ser444) |
| 中文名称 | 磷酸化肌细胞特异性增强因子2D抗体 |
| 别 名 | MEF2D (phospho S444); p-MEF2D (phospho S444); MEF2D(phospho Ser444); DKFZp686I1536; MADS box transcription factor 2 polypeptide D; Mef2d; MEF2D_HUMAN; myocyte enhancer factor 2D; Myocyte specific enhancer factor 2, polypeptide D; Myocyte specific enhancer factor 2D; myocyte-specific enhancer factor 2D. |
DATASHEET
Host:Rabbit
Target Protein:phospho-MEF2D (Ser444)
IR:Immunogen Range:PV(p-S)PS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4209
Swiss Prot:Q14814
Source:KLH conjugated Synthesised phosphopeptide derived from human MEF2D around the phosphorylation site of Ser444:PV(p-S)PS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:MEF2D is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(0.2ug/test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- phospho-MEF2D (Ser444) (bs-5502R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-phospho-MEF2D(Ser444) antibody (bs-5502R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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