Anti-phospho-c-Raf (Ser471) antibody
| 英文名称 | phospho-c-Raf (Ser471) |
| 中文名称 | 磷酸化原癌基因c-Raf抗体 |
| 别 名 | RAF1(phospho Ser471); RAF1(phospho S471); RAF1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); v-raf-1 murine leukemia viral oncogene homolog 1; cRaf; C-RAF; proto-oncogene c-RAF; 6430402F14Rik; AA990557; BB129353; c-Raf; Craf1; D830050J10Rik; MGC102375; Raf-1; Raf 1; v-Raf; c Raf; Craf 1 transforming gene; Craf1 transforming gene; EC 2.7.11.1; Murine sarcoma 3611 oncogene 1; RAF; Raf proto oncogene serine/threonine protein kinase; vraf1 murine leukemia viral oncogene homolog 1; c-Raf. |
DATASHEET
Host:Rabbit
Target Protein:phospho-c-Raf (Ser471)
IR:Immunogen Range:MK(p-S)NN
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5894
Swiss Prot:P04049
Source:KLH conjugated Synthesised phosphopeptide derived from human c-Raf around the phosphorylation site of Ser471:MK(p-S)NN
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-phospho-c-Raf(Ser471) (bs-5649R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 73 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-c-Raf(Ser471)) Polyclonal Antibody, Unconjugated (bs-5649R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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