Anti-Hyaluronidase3 antibody (bs-5889R)

Anti-Hyaluronidase3 antibody

DATASHEET

Host:Rabbit

Target Protein:Hyaluronidase3

IR:Immunogen Range:51-150/417

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:8372

Swiss Prot:O43820

Source:KLH conjugated synthetic peptide derived from human Hyaluronidase3:51-150/417 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:HYAL3 is a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Mouse(Kidney) Lysate at 40 ug
Primary: Anti- Hyaluronidase3 (bs-5889R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD

Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-Hyaluronidase3 antibody (bs-5889R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.