Anti-phospho-TCF3 (Ser39) antibody
| 英文名称 | phospho-TCF3 (Ser39) |
| 中文名称 | 磷酸化转录因子3抗体 |
| 别 名 | TCF3/E2A(phospho Ser39); p-TCF3(phospho Ser39); p-E47(phospho Ser39); p-E47(phospho Ser39); bHLHb21; Class B basic helix-loop-helix protein 21; E12; E2A; E2A immunoglobulin enhancer binding factors E12/E47; E47; Helix loop helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; immunoglobulin transcription factor 1; ITF1; Kappa E2 binding factor; Kappa-E2-binding factor; MGC129647; MGC129648; Negative vitamin D response element binding protein; TCF-3; TCF3; TFE2; TFE2_MOUSE; transcription factor 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDIR; VDR interacting repressor. |
DATASHEET
Host:Rabbit
Target Protein:phospho-TCF3 (Ser39)
IR:Immunogen Range:PA(p-S)LA
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6929
Swiss Prot:P15923
Source:KLH conjugated synthesised phosphopeptide derived from human TCF3 around the phosphorylation site of Ser39:PA(p-S)LA
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Embryo (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-phospho-TCF3 (Ser39) (bs-4662R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 67 kD
Observed band size: 68 kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-TCF3/E2A/E47(Ser39) Polyclonal Antibody, Unconjugated(bs-4662R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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