Anti-RERG antibody (bs-6091R)

Anti-RERG antibody

DATASHEET

Host:Rabbit

Target Protein:RERG

IR:Immunogen Range:101-199/199

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:85004

Swiss Prot:Q96A58

Source:KLH conjugated synthetic peptide derived from human RERG:101-199/199 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:RERG, a member of the RAS superfamily of GTPases, inhibits cell proliferation and tumor formation

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- RERG (bs-6091R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 25 kD

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RERG) Polyclonal Antibody, Unconjugated (bs-6091R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.