Anti-ZNF185 antibody (bs-6119R)

Anti-ZNF185 antibody

DATASHEET

Host:Rabbit

Target Protein:ZNF185

IR:Immunogen Range:551-650/689

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7739

Swiss Prot:O15231

Source:KLH conjugated synthetic peptide derived from human ZNF185:551-650/689 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ZNF185 encodes a LIM domain zinc finger protein. It may be involved in the regulation of cellular proliferation and/or differentiation.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(3ug/Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
MCF-7(Human) Cell Lysate at 40 ug
A431(Human) Cell Lysate at 40 ug
U2os(Human) Cell Lysate at 40 ug
Primary: Anti-ZNF185 (bs-6119R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76/39 kD
Observed band size: 67 kD

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-6199R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.