Anti-SHC3 antibody
| 英文名称 | SHC3 |
| 中文名称 | SH2结构域蛋白C3抗体 |
| 别 名 | N SHC; N-Shc; Neuronal Shc; NSHC; Protein Rai; Rai; SH2 domain protein C3; SHC (Src homology 2 domain containing) transforming protein 3; SHC protein C; SHC-like protein, neuronal; SHC-transforming protein 3; SHC-transforming protein C; Shc3; SHC3_HUMAN; SHCC; Src homology 2 domain-containing transforming protein; Src homology 2 domain-containing-transforming protein C3. |
DATASHEET
Host:Rabbit
Target Protein:SHC3
IR:Immunogen Range:501-594/594
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:53358
Swiss Prot:Q92529
Source:KLH conjugated synthetic peptide derived from human SHC3:501-594/594
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- SHC3 (bs-6199R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Blank control (Black line): A431(Black).
Primary Antibody (green line): Rabbit Anti-ZNF185 antibody (bs-6119R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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