Anti-Prostatic Acid Phosphatase antibody (bs-6293R)

Anti-Prostatic Acid Phosphatase antibody 

DATASHEET

Host:Rabbit

Target Protein:Prostatic Acid Phosphatase

IR:Immunogen Range:21-120/386

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:55

Swiss Prot:P24666

Source:KLH conjugated synthetic peptide derived from human Prostatic Acid Phosphatase:21-120/386 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Prostatic Acid Phosphatase is Belongs to the histidine acid phosphatase family.Cellular localization;Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: PC3 Cell (Human) Lysate at 40 ug
Primary: Anti-Prostatic Acid Phosphatase (bs-6293R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 48 kD

Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Prostatic Acid Phosphatase) Polyclonal Antibody, Unconjugated (bs-6293R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.