Anti-HSD17B6 antibody
| 英文名称 | HSD17B6 |
| 中文名称 | 17-β-羟脱氢酶6抗体 |
| 别 名 | 17-beta-HSD 6; 17-beta-HSD6; 17-beta-hydroJNCsteroid dehydrogenase type 6; 3 hydroJNCsteroid epimerase; 3(alpha >beta) hydroJNCsteroid epimerase; 3(alpha >beta) hydroJNCsteroid epimerasel; 3-alpha->beta-HSE; 3-alpha->beta-hydroJNCsteroid epimerase; H17B6_HUMAN; HSD17B6; HSE; HydroJNCsteroid (17 beta) dehydrogenase 6; HydroJNCsteroid (17 beta) dehydrogenase 6 homolog (mouse); HydroJNCsteroid 17 beta dehydrogenase 6; NAD+ dependent 3 alpha hydroJNCsteroid dehydrogenase 3 hydroJNCsteroid epimerase; NAD+ dependent 3 alpha hydroJNCsteroid dehydrogenase; Oxidative 3 alpha hydroJNCsteroid dehydrogenase; Oxidative 3-alpha hydroJNCsteroid dehydrogenase; Oxidoreductase; Retinol dehydrogenase; RODH; SDR9C6;Short chain dehydrogenase/reductase family 9C, member 6. |
DATASHEET
Host:Rabbit
Target Protein:HSD17B6
IR:Immunogen Range:61-160/317
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:8630
Swiss Prot:O14756
Source:KLH conjugated synthetic peptide derived from human HSD17B6:61-160/317
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. [provided by RefSeq, Aug 2013]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSD17B6 Antibody(bs-6592R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-HSD17B6 antibody (bs-6592R)
Dilution:3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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