Anti-NALP2 antibody
| 英文名称 | NALP2 |
| 中文名称 | 富含亮氨酸重复结构域蛋白2抗体 |
| 别 名 | CLR19.9; FLJ20510; Leucine rich repeat and PYD containing protein 2; LRR and PYD domains-containing protein 2; NACHT; NACHT domain; NACHT leucine rich repeat and PYD containing 2; NACHT LRR and PYD domains containing protein 2; NALP2; NALP2_HUMAN; NBS1; NLR family pyrin domain containing 2; NLRP2; Nucleotide binding oligomerization domain leucine rich repeat and pyrin domain containing 2; Nucleotide-binding site protein 1; PAN1; PYPAF2; PYRIN containing APAF1 like protein2; PYRIN domain and NACHT domain-containing protein 1; PYRIN-containing APAF1-like protein 2. |
DATASHEET
Host:Rabbit
Target Protein:NALP2
IR:Immunogen Range:851-1062/1062
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:55655
Swiss Prot:Q9NX02
Source:KLH conjugated synthetic peptide derived from human NACHT, LRR and PYD domains-containing protein 2:851-1062/1062
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:NALP proteins, such as NALP2, are characterized by an N-terminal pyrin (MIM 608107) domain (PYD) and are involved in the activation of caspase-1 (CASP1; MIM 147678) by Toll-like receptors (see TLR4; MIM 603030). They may also be involved in protein complexes that activate proinflammatory caspases (Tschopp et al., 2003 [PubMed 12563287]).[supplied by OMIM, Mar 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Thymus(Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
Primary: Anti-NALP2 (bs-6717R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 117 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NALP2/PAN1/PYPAF2 Polyclonal Antibody, Unconjugated(bs-6717R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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