Anti-5HT2C Receptor antibody
| 英文名称 | 5HT2C Receptor |
| 中文名称 | 5-羟色胺受体2C抗体 |
| 别 名 | 5 HydroJNCtryptamine 2C receptor; 5-HT-1C; 5-ht-1c receptor; 5-HT-2C; 5-HT1C; 5-HT2C; 5-HTR2C; 5-hydroJNCtryptamine (serotonin) receptor 2C, G protein-coupled; 5-hydroJNCtryptamine receptor 1C; 5-hydroJNCtryptamine receptor 2C; 5HT1C; 5HT2C; 5HT2C_HUMAN; 5HTR2C; 5HydroJNCtryptamine 2C receptor; Htr1c; HTR2C; serotonin 1c receptor; serotonin 2c receptor; Serotonin 5-HT-2C receptor; Serotonin receptor 2C. |
DATASHEET
Host:Rabbit
Target Protein:5HT2C Receptor
IR:Immunogen Range:311-410/458
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3358
Swiss Prot:P28335
Source:KLH conjugated synthetic peptide derived from human 5HTR2C:311-410/458
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Serotonin (5-hydroJNCtryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Raji Cell (Human) Lysate at 40 ug
Primary: Anti-5HTR2C (bs-2959R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HTR2C) Polyclonal Antibody, Unconjugated (bs-2959R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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