Anti-EphA3 antibody
| 英文名称 | EphA3 |
| 中文名称 | 酪氨酸蛋白激酶受体A3抗体 |
| 别 名 | EK4; End3; Eph receptor A3; EPH-like kinase 4; EPH-like tyrosine kinase 1; EphA3; Ephrin receptor EphA3; Ephrin type-A receptor 3; ETK 1; ETK; ETK1; HEK 4; HEK; Hek4; Human embryo kinase; Mek4; MGC109882; Receptor tyrosine kinase HEK; Tyro 4; Tyro4; Tyrosine protein kinase receptor ETK 1; Tyrosine-protein kinase TYRO4; AW492086; Cek4; EC 2.7.10.1; EPHA3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:EphA3
IR:Immunogen Range:401-500/983
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2042
Swiss Prot:P29320
Source:KLH conjugated synthetic peptide derived from human EphA3/Eph receptor A3:401-500/983
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:EphA3 is a member of the Ephrin (Eph) family. The Ephrins and Eph-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the Ephrin A (EphA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the Ephrin B (EphB) class, which are transmembrane proteins. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
H9C2(Rat) Cell Lysate at 30 ug
Primary: Anti-EphA3 (bs-7032R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106 kD
Observed band size: 106 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EphA3) Polyclonal Antibody, Unconjugated (bs-7032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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