Anti-phospho-Eph receptor B1 (Tyr928) antibody
| 英文名称 | phospho-Eph receptor B1 (Tyr928) |
| 中文名称 | 磷酸化酪氨酸蛋白激酶受体B1抗体 |
| 别 名 | Eph receptor B1(phospho Y928); EphB1(phospho Y928); Cek 6; Elkh; Eph tyrosine kinase 2; EPH2; EphB1; Ephrin receptor Eph B1 precursor; Ephrin type B receptor 1; HEK 6; NET; Neuronally expressed EPH related tyrosine kinase; Tyrosine protein kinase receptor EPH 2; ELK; EPH-like kinase 6; Ephb1; EPHB1_HUMAN; Ephrin type B receptor 1; Ephrin type-B receptor 1; EPHT2; HEK6; Neuronally-expressed EPH-related tyrosine kinase; Tyrosine protein kinase receptor EPH 2; Tyrosine-protein kinase receptor EPH-2. |
DATASHEET
Host:Rabbit
Target Protein:phospho-Eph receptor B1 (Tyr928)
IR:Immunogen Range:VQ(p-Y)RD
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2047
Swiss Prot:P54762
Source:KLH conjugated Synthesised phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr928:VQ(p-Y)RD
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:phrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti-phospho-Eph receptor B1 (Tyr928) (bs-10138R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106 kD
Observed band size: 121 kD
Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-EphB1/Eph receptor B1(Tyr928) Polyclonal Antibody, Unconjugated (bs-10138R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
好评度