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Anti-phospho-Eph receptor B1 (Tyr968) antibody (bs-10139R)

Anti-phospho-Eph receptor B1 (Tyr968) antibody (bs-10139R)

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Anti-phospho-Eph receptor B1 (Tyr968) antibody

英文名称phospho-Eph receptor B1 (Tyr968)
中文名称磷酸化酪氨酸蛋白激酶受体B1抗体
别    名Eph receptor B1(phospho Y968); EphB1(phospho Y968)Cek 6; Elkh; Eph tyrosine kinase 2; EPH2; EphB1; Ephrin receptor Eph B1 precursor; Ephrin type B receptor 1; HEK 6; NET; Neuronally expressed EPH related tyrosine kinase; Tyrosine protein kinase receptor EPH 2.  

DATASHEET

Host:Rabbit

Target Protein:phospho-Eph receptor B1 (Tyr968)

IR:Immunogen Range:LN(p-S)I-NH2

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2047

Swiss Prot:P54762

Source:KLH conjugated Synthesised phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr968:LN(p-S)I-NH2 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Eph receptor B1 (Tyr968)) Polyclonal Antibody, Unconjugated (bs-10139R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Eph receptor B1 (Tyr968)) Polyclonal Antibody, Unconjugated (bs-10139R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

 

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