Anti-PEF1 antibody (bs-6768R)

Anti-PEF1 antibody

DATASHEET

Host:Rabbit

Target Protein:PEF1

IR:Immunogen Range:121-230/284

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:553115

Swiss Prot:Q9UBV8

Source:KLH conjugated synthetic peptide derived from human PEF1:121-230/284 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PEF1 (penta-EF hand domain containing 1) is a Ca(2+)-binding protein that belongs to the penta-EF hand family which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca(2+).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1ug/Test)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Sheep
Monkey
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Intestine (Mouse) Lysate at 40 ug
Primary: Anti-PEF1 (bs-6768R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD

Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).