Anti-phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596) antibody (bs-7554R)

Anti-phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596)

IR:Immunogen Range:KT(p-Y)VD

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1969

Swiss Prot:P29317

Source:KLH conjugated synthesised phosphopeptide derived from human Eph receptor A2 around the phosphorylation site of Tyr588 + Tyr596:KT(p-Y)VD 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Eph receptor A2 is the receptor for members of the ephrin-A family. It binds to ephrin-A1, -A3, -A4 and -A5. Eph receptor A3 is also a receptor for members of the ephrin-A family. It binds to ephrin-A2, -A3, -A4 and -A5 and is thought to play a role in lymphoid function. Eph receptor A4 is a receptor for members of the ephrin-A family. It binds to ephrin-A1, -A4 and -A5. It binds more poorly to ephrin-A2 and -A3. It may play a role in a signal transduction process involved in hindbrain pattern formation.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Liver (Mouse)Lysate at 40 ug
Primary: Anti-phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596)(bs-7554R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 105kD
Observed band size: 130kD

Sample:
293T Cell (Human) Lysate at 30 ug
Raji Cell (Human) Lysate at 30 ug
Primary:Anti-p-Eph receptorA2+A3+A4(Tyr588+Tyr596) (bs-7554R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 105 kD
Observed band size: 135 kD