Anti-Flightless 1 antibody
| 英文名称 | Flightless 1 |
| 中文名称 | 凝溶胶蛋白家族Fli-1/Flightless I抗体 |
| 别 名 | Fli 1; FLI; Fli1; Flightless-1; Flightless1; Flightless 1; Flightless I (Drosophila) homolog; Flightless I homolog; Flightless I homolog (Drosophila); Flightless1; FlightlessI; FLII; Fliih; FLIL; MGC39265; Protein flightless 1 homolog; FLI1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Flightless 1
IR:Immunogen Range:343-452/452
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2314
Swiss Prot:Q13045
Source:KLH conjugated synthetic peptide derived from human Flightless 1:343-452/452
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene leads to abnormal muscle function, arrested development and embryonic lethality. The protein sequence shows that this might be a regulator of cytoskeleton and may have a role during cell division.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Flightless 1 (bs-7864R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Flightless 1) Polyclonal Antibody, Unconjugated (bs-7864R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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