Anti-ICMT antibody (bs-8019R)

Anti-ICMT antibody

DATASHEET

Host:Rabbit

Target Protein:ICMT

IR:Immunogen Range:211-278/284

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23463

Swiss Prot:O60725

Source:KLH conjugated synthetic peptide derived from human CMT/Icmt:211-278/284 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Tissue specificity: Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICMT) Polyclonal Antibody, Unconjugated (bs-8019R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: human pancreatic carcinoma(BXPC-3);4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ICMT Polyclonal Antibody, Unconjugated(bs-8019R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei