Anti-PANK1 antibody (bs-8337R)

Anti-PANK1 antibody

DATASHEET

Host:Rabbit

Target Protein:PANK1

IR:Immunogen Range:501-598/598

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:53354

Swiss Prot:Q8TE04

Source:KLH conjugated synthetic peptide derived from human PANK1:501-598/598 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The pantothenate kinase (PANK) family of proteins catalyzes the first step in coenzyme A (CoA). Pantothenate kinase 1 (PANK1) is a 598 amino acid member of the pantothenate kinase family that plays a role in the physiological regulation of the intracellular CoA concentration. Localized to the cytoplasm, PANK1 is strongly inhibited by acetyl-CoA and manyl-CoA, as well as by high concentration of non-esterified CoA (CoASH). Four known isoforms of PANK1 exist as a result of alternative splicing events. Of these isoforms, PANK1a and PANK1b have been identified as the catalytically active isoforms. Isoform PANK1a is most highly expressed in brain, heart, kidney, liver, skeletal muscle and kidney. Isoform PANK1b is detected at much lower levels in kidney, liver, brain and testis and is not detected in heart or skeletal muscle.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Cerebellum (Rat) Lysate at 40 ug
Primary: Anti-PANK1 (bs-8337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60/50 kD
Observed band size: 60/50 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PANK1) Polyclonal Antibody, Unconjugated (bs-8337R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.