Anti-BTNL2 antibody (bs-8431R)

Anti-BTNL2 antibody

DATASHEET

Host:Rabbit

Target Protein:BTNL2

IR:Immunogen Range:24-120/455

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:56244

Swiss Prot:Q9UIR0

Source:KLH conjugated synthetic peptide derived from human BTNL2:24-120/455 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Butyrophilin is a glycoprotein that is specifically expressed on the apical surface of mammary epithelial cells during lactation and becomes incorporated as an integral protein into the membrane of the milk fat globule during the budding and secretion of fat droplets into milk. BTNL2 (butyrophilin-like protein 2), also known as BTL-II, is a 455 amino acid single-pass type II membrane protein that acts as a negative regulator of T cell proliferation. Expressed in thymus, brain, kidney, heart, liver, small intestine, testis, ovary, pancreas and leukocytes, BTNL2 exists as at least six alternatively spliced isoforms, which are encoded by a gene located on human chromosome 6. Expression of BTNL2 isoform 3 is associated with susceptibility to sarcoidosis type 2 (SS2), an immune disorder that causes chronic inflammatory granulomatous lesions.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-BTNL2 (bs-8431R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (BTNL2) Polyclonal Antibody, Unconjugated (bs-8431R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.