Anti-phospho-HNF4 (Ser304) antibody
| 英文名称 | phospho-HNF4 (Ser304) |
| 中文名称 | 磷酸化肝细胞核因子4α抗体 |
| 别 名 | HNF4(Phospho-Ser304); Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; HNF 4 alpha; HNF 4; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF 14; TCF; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF4; HNF4A_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-HNF4 (Ser304)
IR:Immunogen Range:GL(p-S)DP
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3172
Swiss Prot:P41235
Source:KLH conjugated Synthesised phosphopeptide derived from human HNF4 alpha around the phosphorylation site of Ser304:GL(p-S)DP
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Horse
Rabbit
Guinea Pig
Danio rerio.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-phospho-HNF4 (Ser304) (bs-8451R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HNF4) Polyclonal Antibody, Unconjugated (bs-8451R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
好评度