Anti-CD71/Transferrin receptor antibody (bs-0988R)

Anti-CD71/Transferrin receptor antibody

DATASHEET

Host:Rabbit

Target Protein:CD71/Transferrin receptor

IR:Immunogen Range:621-720/760

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7037

Swiss Prot:P02786

Source:KLH conjugated synthetic peptide derived from human Transferrin receptor:621-720/760 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1μg /Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-CD71/Transferrin receptor (bs-0988R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74/85 kD
Observed band size: 75 kD

Blank control(blue):Raji(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti- CD71 antibody(bs-0988R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-CD71 antibody (bs-0988R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.