Anti-SEMA4D antibody
| 英文名称 | SEMA4D |
| 中文名称 | 跨膜蛋白SEMA4D抗体 |
| 别 名 | A8 antibody BB 18; BB18; CD 100; CD100; CD100 antigen; Coll 4; COLL4; Collapsin 4; Collapsin4; GR3; Human semaphorin CD100 mRNA complete cds; Leukocyte activation antigen CD100; M sema G; MSEMA; SEM4D_HUMAN; Sema 4d; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D ; Sema H; SEMA J; SEMA4D; Semacl 2; Semacl2; SemaH; SEMAJ; Semaphorin 4D; Semaphorin 4D precursor; Semaphorin C like 2; Semaphorin H; Semaphorin J; Semaphorin-4D; Semaphorin4D; SemaphorinJ; Semcl 2; Semcl2. |
DATASHEET
Host:Rabbit
Target Protein:SEMA4D
IR:Immunogen Range:75-170/862
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10507
Swiss Prot:Q92854
Source:KLH conjugated synthetic peptide derived from human SEMA4D:75-170/862
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:May play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1μg /Test)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-SEMA4D (bs-6965R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60/126 kD
Observed band size: 60 kD
Blank control: Raji(blue).
Primary Antibody:Rabbit Anti-SEMA4D antibody(bs-6965R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-6965R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
好评度