Anti-Kininogen-1 light chain antibody (bs-9504R)

Anti-Kininogen-1 light chain antibody

DATASHEET

Host:Rabbit

Target Protein:Kininogen-1 light chain

IR:Immunogen Range:551-644/644

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3827

Swiss Prot:P01042

Source:KLH conjugated synthetic peptide derived from human HMW Kininogen-1 light chain:551-644/644 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
liver(mouse) Lysate at 40 ug
Primary: Anti-Kininogen-1 light chain (bs-9504R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28/70kD
Observed band size: 28 kD

Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti- Kininogen-1 light chain (bs-9504R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD