Anti-phospho-APE (Ser1417) antibody
| 英文名称 | phospho-APE (Ser1417) |
| 中文名称 | 磷酸化肌动蛋白结合蛋白Girdin抗体 |
| 别 名 | Girdin (phospho S1417); p-APE(Ser1417); Akt phosphorylation enhancer; APE; Coiled coil domain containing protein 88A; G alpha interacting vesicle associated protein; Girders of actin filament; GIV; HkRP1; Hook related protein 1; AKT iphosphorylation enhancer; Akt phosphorylation enhancer; Ccdc88a; GIV; GRDN; GRDN_HUMAN; HkRP1; Hook related protein 1; Hook-related protein 1; KIAA1212. |
DATASHEET
Host:Rabbit
Target Protein:phospho-APE (Ser1417)
IR:Immunogen Range:QK(p-S)LT
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:55704
Swiss Prot:Q3V6T2
Source:KLH conjugated synthesised phosphopeptide derived from human APE around the phosphorylation site of Ser1417:QK(p-S)LT
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1ug/test)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-APE(Ser1417) (bs-6465R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 206 kD
Observed band size: 206 kD
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-phospho-APE(Ser1417) antibody (bs-6465R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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