Anti-ARHGAP32 antibody
| 英文名称 | ARHGAP32 |
| 中文名称 | Rho GTP酶激活蛋白32抗体 |
| 别 名 | Brain-specific Rho GTPase-activating protein; GAB-associated Cdc42/Rac GTPase-activating protein; GC-GAP; GRIT; GTPase regulator interacting with TrkA; p200RhoGAP; p250GAP; PX-RICS; rac GTPase activating protein; Rho GTPase activating protein 32; Rho GTPase-activating protein 32; Rho-type GTPase-activating protein 32; Rho/Cdc42/Rac GTPase-activating protein RICS; RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling; RICS. |
DATASHEET
Host:Rabbit
Target Protein:ARHGAP32
IR:Immunogen Range:801-920/2078
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9743
Swiss Prot:A7KAX9
Source:KLH conjugated synthetic peptide derived from human ARHGAP32:801-920/2078
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:ARHGAP32 is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ARHGAP32 Polyclonal Antibody, Unconjugated(bs-9296R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-ARHGAP32 antibody (bs-9296R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
好评度