Anti-NHEDC2 antibody (bs-9712R)

Anti-NHEDC2 antibody

DATASHEET

Host:Rabbit

Target Protein:NHEDC2

IR:Immunogen Range:151-250/537

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:133308

Swiss Prot:Q86UD5

Source:KLH conjugated synthetic peptide derived from human NHEDC2:151-250/537 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Na+/H+ exchangers (NHEs) catalyze the transport of Na+ in exchange for H+ across membranes in organisms and are required for numerous physiological processes. NHEDC2 (Na+/H+ exchanger-like domain-containing protein 2), also known as NHA2, is a 537 amino acid mitochondrial protein. NHEDC2 is involved in organelle volume homeostasis by catalyzing the exchange of protons for Na+ and Li+ across the inner mitochondrial membrane. Found in red blood cells, NHEDC2 is required for bone resorption activity and osteoclast differentiation. As a multi-pass membrane protein, NHEDC2 is expressed as two isoforms produced by alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: HL60 (Human) Cell Lysate at 30 ug
Lane 2: Hcclm3 (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: U937 (Human) Cell Lysate at 30 ug
Primary: Anti-NHEDC2 (bs-9712R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Sample:
Spinal cord (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- NHEDC2 (bs-9712R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD