Anti-HSPBAP1 antibody (bs-6986R)

Anti-HSPBAP1 antibody

DATASHEET

Host:Rabbit

Target Protein:HSPBAP1

IR:Immunogen Range:151-250/488

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:79663

Swiss Prot:Q96EW2

Source:KLH conjugated synthetic peptide derived from human HSPBAP1:151-250/488 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Heat Shock Protein BAP1 (heat shock 27-kDa-associated protein 1, or HSPBAP1) inhibits the function of heat shock protein 27, which has a neuroprotective effect during experimentally induced epileptic neuropathology. The HSPBAP1 protein, also known as PASS1, contains a jmjC motif, suggesting it is a member of the jumonji family of transcription factors. There is evidence of domain swapping within the jumonji family genes, and the HSPBAP1 gene has been shown to fuse with a novel gene called DIRC3 in hereditary renal cell cancer.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
293T Cell(human) Lysate at 40 ug
Lymph node (mouse) Lysate at 40 ug
HepG2 Cell(human) Lysate at 40 ug
Testis (mouse) Lysate at 40 ug
Primary: Anti- HSPBAP1(bs-6986R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55 kD

Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated (bs-6986R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.