Anti-p70 S6 kinase beta antibody
| 英文名称 | p70 S6 kinase beta |
| 中文名称 | 核糖体蛋白S6激酶1抗体 |
| 别 名 | 70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1; KS6B2_HUMAN; p70 beta; p70 ribosomal S6 kinase beta; p70 S6K-beta; p70 S6KB; p70 S6Kbeta; p70(S6K) beta; p70-beta; p70-S6K 2; P70S6K2; p70S6Kb; Ribosomal protein S6 kinase 70kDa, polypeptide 2; Ribosomal protein S6 kinase beta 2; Ribosomal protein S6 kinase beta-2; Rps6kb2; S6 kinase related kinase; S6 kinase-related kinase; S6K beta 2; S6K beta; S6K-beta; S6K-beta-2; S6K2; Serine/threonine protein kinase 14 beta; Serine/threonine-protein kinase 14B; SRK; STK14B. |
DATASHEET
Host:Rabbit
Target Protein:p70 S6 kinase beta
IR:Immunogen Range:281-380/525
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6199
Swiss Prot:Q9UBS0
Source:KLH conjugated synthetic peptide derived from human p70 S6 kinase beta:281-380/525
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:B16 (Mouse)Cell Lysate at 40 ug
Primary: Anti-p70 S6 kinase beta(bs-4867R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-p70 S6 kinase beta Polyclonal Antibody, Unconjugated(bs-4867R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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