Anti-phospho-LEF1 (Ser42) antibody
| 英文名称 | phospho-LEF1 (Ser42) |
| 中文名称 | 磷酸化淋巴增强因子-1抗体 |
| 别 名 | LEF1 (phospho S42); LEF1 (phospho Ser42); p-LEF1(phospho S42); p-LEF1(Ser42); lymphoid enhancing factor-1; Transcription factor T cell specific 1 alpha ; DKFZp586H0919; LEF 1; LEF1; Lymphoid enhancer binding factor 1; T cell specific transcription factor 1 alpha; TCF 1 alpha; TCF1 alpha; TCF1alpha; TCF1-alpha; LEF1_HUMAN; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha. |
DATASHEET
Host:Rabbit
Target Protein:phospho-LEF1 (Ser42)
IR:Immunogen Range:EI(p-S)HP
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:51176
Swiss Prot:Q9UJU2
Source:KLH conjugated synthesised phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42:EI(p-S)HP
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-LEF1 (Ser42) (bs-9842R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 58 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-LEF1(Ser42) Polyclonal Antibody, Unconjugated(bs-9842R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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