Anti-WIPF2 antibody (bs-9869R)

Anti-WIPF2 antibody

DATASHEET

Host:Rabbit

Target Protein:WIPF2

IR:Immunogen Range:31-130/440

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:147179

Swiss Prot:Q8TF74

Source:KLH conjugated synthetic peptide derived from human WIPF2:31-130/440 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:WIRE, also known as WIPF2 (WAS/WASL interacting protein family, member 2) or WICH, is a 440 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one WH2 domain. Expressed ubiquitously with highest expression in colon, brain, lung and stomach, WIRE functions as an N-WASP-interacting protein that plays an important role in the organization and mobilization of the Actin cytoskeleton. Additionally, WIRE is involved in the formation of cell surface protrusions and may also provide a link between the cytoskeletal machinery and PDGF-B receptors. Multiple alternatively spliced isoforms of WIRE exist and are encoded by a gene that maps to human chromosome 17.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: A549 Cell (Human) Lysate at 30 ug
Primary: Anti- WIPF2 (bs-9869R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46kD
Observed band size: 46kD

Paraformaldehyde-fixed, paraffin embedded (rat bain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WIPF2) Polyclonal Antibody, Unconjugated (bs-9869R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining