Anti-phospho-IKKi (Ser172) antibody
| 英文名称 | phospho-IKKi (Ser172) |
| 中文名称 | 磷酸化核因子NFκB抑制蛋白激酶i抗体 |
| 别 名 | IKKi/IKKe (phospho Ser172); p-IKKi(Ser172); p-IKKe(Ser172); IKKi/IKKe; I kappa B kinase epsilon; IkBKE; IKBKE protein; IKK E; IKK i; IKK-i; IKK related kinase epsilon; IKKE; IKK-E; IKKepsilon; IKKI; Inducible I kappa B kinase; inducible IkappaB kinase; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon; Inhibitor of nuclear factor kappa B kinase subunit epsilon; KIAA0151; MGC125294; MGC125295; MGC125297; IKKE_HUMAN; I-kappa-B kinase epsilon; IKK-epsilon; Inducible I kappa-B kinase. |
DATASHEET
Host:Rabbit
Target Protein:phospho-IKKi (Ser172)
IR:Immunogen Range:FV(p-S)VY
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9641
Swiss Prot:Q14164
Source:KLH conjugated synthesised phosphopeptide derived from human IKKi/IKKE around the phosphorylation site of Ser172:FV(p-S)VY
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Spleen (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- phospho-IKKi (Ser172) (bs-8583R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 71 kD
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-IKKi(Ser172) Polyclonal Antibody, Unconjugated(bs-8583R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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