Anti-Osteocalcin antibody
| 英文名称 | Osteocalcin |
| 中文名称 | 骨钙蛋白/骨钙素抗体 |
| 别 名 | Osteocalcin Propeptide; Osteocalcin; Osteocalcin precursor; Gamma-carboJNCglutamic acid-containing protein; Bone Gla-protein; BGLAP; Bone gamma carboJNCglutamate protein; BGLAP; BGP; Bone gamma carboJNCglutamate gla protein osteocalcin; Bone gamma carboJNCglutamate protein; Bone Gla protein; Gamma carboJNCglutamic acid containing protein; OC; PMF1; OSTCN_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Osteocalcin
IR:Immunogen Range:21-100/100
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:632
Swiss Prot:P02818
Source:KLH conjugated synthetic peptide derived from human Osteocalcin:21-100/100
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboJNClase produces three gamma carboJNCglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin (bs-0437P-Cy3) at[1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.
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