Anti-KIF5A/NKHC1 antibody
| 英文名称 | KIF5A/NKHC1 |
| 中文名称 | 驱动蛋白KIF5A抗体 |
| 别 名 | Kinesin 5A; Kinesin5A; Kinesin-5A; KIF 5A; KIF5A; KIF5A_HUMAN; Kinesin family member 5A; Kinesin heavy chain isoform 5A; Kinesin Heavy Chain Neuron Specific; Kinesin heavy chain neuron-specific 1; neuronal kinesin heavy chain 1; MY050; Neuronal kinesin heavy chain; NKHC 1; NKHC1; NKHC-1; NKHC; SPG 10. |
DATASHEET
Host:Rabbit
Target Protein:KIF5A/NKHC1
IR:Immunogen Range:201-300/1032
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3798
Swiss Prot:Q12840
Source:KLH conjugated synthetic peptide derived from human KIF5A/NKHC1:201-300/1032
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:NKHC1 is a neuronal-specific component of a multi-subunit “molecular motor” complex that mediates intracellular organelle transport. Mutations in the gene encoding NKHC1 cause autosomal dominant spastic paraplegia 10. NKHC1 has a pan-neuronal distribution in the nervous system. Rat tissue extracts by immunoblot of NKHC1 can produce a doublet only in brain and sciatic nerve tissue. NKHC1 is distributed throughout the central nervous system and is enriched in subsets of neurons. Within cultured hippocampal neurons, NKHC1 is concentrated in the perinuclear region of the cell body. Kinesin superfamily proteins like NKHC1 are the molecular motors conveying cargos along microtubules.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIF5A/NKHC1) Polyclonal Antibody, Unconjugated (bs-11027R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIF5A/NKHC1) Polyclonal Antibody, Unconjugated (bs-11027R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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