Anti-Spindly antibody
| 英文名称 | Spindly |
| 中文名称 | 卷曲螺旋结构域蛋白99抗体 |
| 别 名 | Rrhabdomyosarcoma antigen protein MU RMS 40.4A; Arsenite related gene 1 protein; Arsenite-related gene 1 protein; CCDC 99; Ccdc99; Coiled coil domain containing 99; Coiled-coil domain-containing protein 99; hSpindly; Protein Spindly; rhabdomyosarcoma antigen MU RMS 40.4A; Rhabdomyosarcoma antigen MU-RMS-40.4A; SPDLY_HUMAN; SPDL1. |
DATASHEET
Host:Rabbit
Target Protein:Spindly
IR:Immunogen Range:401-500/605
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:54908
Swiss Prot:Q96EA4
Source:KLH conjugated synthetic peptide derived from human Protein Spindly:401-500/605
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The five integral families of plant hormones consists of auxins, cytokinins, giberellins (GAs), abscisic acid (ABA), and ethylene. Giberellins, which consist of over fifty family members, mediate shoot growth. In Arabidopsis, SPINDLY (SPY) negatively regulates GA signal transduction. ERA1 (enhanced response to absisic acid), which is identical to WIGGUM, controls floral and shoot apical meristem size and floral organ number in response to ABA. Ethylene is perceived by a family of five receptors, one of which is ETR1, whereas CTR1 is a negative regulator of the ethylene signal transduction pathway . Ethylene is also produced endogenously in Arabidopsis via a biosynthetic pathway, which is catalyzed by ACC synthase and ACC oxidase.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Spindly Polyclonal Antibody, Unconjugated(bs-2321R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Spindly Polyclonal Antibody, Unconjugated(bs-2321R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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