Anti-PCDH12 antibody (bs-11109R)

Anti-PCDH12 antibody

DATASHEET

Host:Rabbit

Target Protein:PCDH12

IR:Immunogen Range:401-500/1184

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:51294

Swiss Prot:Q9NPG4

Source:KLH conjugated synthetic peptide derived from human PCDH12:401-500/1184 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 6 cadherin repeats, a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene localizes to the region on chromosome 5 where the protocadherin gene clusters reside. The exon organization of this transcript is similar to that of the gene cluster transcripts, notably the first large exon, but no significant sequence homology exists. The function of this cellular adhesion protein is undetermined but mouse protocadherin 12 does not bind catenins and appears to have no affect on cell migration or growth.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1μg/Test)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
SP2/0(Mouse) Cell Lysate at 30 ug
Primary: Anti-PCDH12 (bs-11109R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD

Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody : Rabbit Anti-CD28 antibody (bs-11109R); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA