Anti-Caspr2 antibody (bs-11129R)

Anti-Caspr2 antibody 

DATASHEET

Host:Rabbit

Target Protein:Caspr2

IR:Immunogen Range:801-900/1331

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:26047

Swiss Prot:Q9UHC6

Source:KLH conjugated synthetic peptide derived from human Caspr2:801-900/1331 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CASPR is a transmembrane glycoprotein of the neurexin superfamily that is highly enriched in regions of myelinated axons. The axons of myelinated nerves in the adult nervous system possess specialized subcellular structures essential for efficient and rapid action potential propagation. CASPR and the closely related molecule CASPR2, a mammalian homolog of Drosophila Neurexin IV (Nrx-IV), demarcate distinct subdomains in myelinated axons. While CASPR is present at the paranodal junctions, CASPR2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region. CASPR2 specifically associates with Kv1.1, Kv1.2, and their Kvbeta2 subunit. CASPR family members may play a role in the local differentiation of the axon into distinct functional subdomains.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(3ug/Test)

Cross Reactive Species:Human
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Caspr2 antibody (bs-11129R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Positive control: RSC96 cells
Concebtration: 5μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.