Anti-Beta-casein antibody (bs-10032R)

Anti-Beta-casein antibody

DATASHEET

Host:Rabbit

Target Protein:Beta-casein

IR:Immunogen Range:16-100/224

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:281099

Swiss Prot:P02666

Source:KLH conjugated synthetic peptide derived from cow Beta-casein:16-100/224 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Milk proteins are crucial for the development of all newborn mammals and caseins constitute the major proteins in mammalian milk. b- and k-caseins are the only caseins present in human milk. The b-casein/k-casein ratio is higher in colostrum than in transitional and mature milk and is related to a better digestibility of colostrum casein micelles by the neonate during the first days of life. Human b-casein-encoding gene (Bca) contains a highly phosphorylated site, which is responsible for the calcium-binding capacity of b-casein. A common set of transcription factors are required for the expression of b-casein. Multiple binding sites for Stat5, C/EBPb (CCAAT/enchancer-binding protein) and several half-sites for glucocorticoid receptor (GR) are identified in the distal human enhancer of the b-casein gene. b-casein gene transcription is regulated primarily by a composite response element (CoRE), which integrates signaling from the lactogenic hormones PRL, insulin and hydrocortisone in mammary epithelial cells. NFkB functions as a negative regulator of b-casein gene expression during pregnancy by interfering with Stat5 tyrosine phosphorylation

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Cow
Sheep
Goat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Milk (Goat) Lysate at 2 ug
Lane 2: Milk (Cow) Lysate at 30 ug
Lane 3: Milk (Cow) Lysate at 3 ug
Lane 4: Milk (Human) Lysate at 30 ug
Primary: Anti-Beta-casein (bs-10032R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25-29 kD
Observed band size: 25-29 kD

Sample:
Lane 1: Milk (Cow) Lysate at 40 ug
Primary: Anti-Beta-casein (bs-10032R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 24 kD