Anti-RNF39 antibody (bs-11393R)

Anti-RNF39 antibody

DATASHEET

Host:Rabbit

Target Protein:RNF39

IR:Immunogen Range:182-230/420

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:80352

Swiss Prot:Q9H2S5

Source:KLH conjugated synthetic peptide derived from human RNF39:182-230/420 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF39 (RING finger protein 39), also known as HZFW, HZF or LIRF, is a 420 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger and one SPRY domain. Expressed in testis, RNF39 is thought to play a role in maintaining prolonged LTP (long term-potentiation, or the process by which synaptic strength continues to increases following chemical stimulation). Via its ability to influence the length of the LTP response, RNF39 functions to regulate early synaptic plasticity. Multiple isoforms of RNF39 exist due to alternative splicing events.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Monkey
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Protein: Raji lysate at 40ug;
Primary: rabbit Anti-RNF39 (bs-11393R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 38/45 kD
Observed band size: 38 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF39) Polyclonal Antibody, Unconjugated (bs-11393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.