Anti-Semaphorin 3E antibody (bs-11480R)

Anti-Semaphorin 3E antibody

DATASHEET

Host:Rabbit

Target Protein:Semaphorin 3E

IR:Immunogen Range:401-450/775

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9723

Swiss Prot:O15041

Source:KLH conjugated synthetic peptide derived from human SEMA3E/Semaphorin 3E:401-450/775 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti- Semaphorin 3E (bs-11480R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kD
Observed band size: 111 kD

Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- Semaphorin 3E (bs-11480R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kD
Observed band size: 111 kD