Anti-Nuclear Pore Complex Proteins/Nup107 antibody (bs-11678R)

Anti-Nuclear Pore Complex Proteins/Nup107 antibody 

DATASHEET

Host:Rabbit

Target Protein:Nuclear Pore Complex Proteins/Nup107

IR:Immunogen Range:501-600/925

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:57122

Swiss Prot:P57740

Source:KLH conjugated synthetic peptide derived from human Nuclear Pore Complex Proteins:501-600/925 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is an essential component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(3μg/Test)

Cross Reactive Species:Human
Mouse
Rat
Pig
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti- Nuclear Pore Complex Proteins'Nup107 (bs-11678R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106kD
Observed band size: 106kD

Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Nuclear Pore Complex Proteins/Nup107/AF488 Conjugated antibody (bs-11678R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.