Anti-Chordin antibody (bs-11831R)

Anti-Chordin antibody

DATASHEET

Host:Rabbit

Target Protein:Chordin

IR:Immunogen Range:412-490/951

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:8646

Swiss Prot:Q9H2X0

Source:KLH conjugated synthetic peptide derived from human Chordin:412-490/951 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The patterning of the CNS relies on the interaction of multiple signaling molecules such as Sonic Hedgehog, Wnts, and BMPs and their antagonists Chordin and Noggin. At midgastrula, expression of Noggin overlaps that of Chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The gene which encodeschordin maps to human chromosome 3q27. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily of signaling proteins, such as BMP-2,4,7. The gene which encodes noggin maps to human chromosome 17q22.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HepG2(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Chordin (bs-11831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 99 kD

Sample:
HepG2(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Chordin (bs-11831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 96 kD