Anti-LGI3 antibody (bs-11879R)

Anti-LGI3 antibody

DATASHEET

Host:Rabbit

Target Protein:LGI3

IR:Immunogen Range:101-220/548

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:203190

Swiss Prot:Q8N145

Source:KLH conjugated synthetic peptide derived from human LGI3:101-220/548 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic ?/∫ horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. Leucine-rich glioma-inactivated protein 3 (LGI3), also known as LGI1-like protein 4 (LGIL4) or leucine-rich repeat LGI family member 3, is a 548 amino acid secretory protein. LGI3 contains five LRR repeats and seven EAR repeats. Widely expressed, with highest levels in brain and lung, LGI3 has been shown to colocalize with endocytosis-associated proteins, lipid raft markers, and Syntaxin. The gene encoding LGI3 maps to chromosome 8p21.3.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- LGI3 (bs-11879R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LGI3) Polyclonal Antibody, Unconjugated (bs-11879R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.