Anti-phospho-E2F1 (Ser337) antibody
| 英文名称 | phospho-E2F1 (Ser337) |
| 中文名称 | 磷酸化转录因子E2F-1抗体 |
| 别 名 | phospho-E2F1(Ser337); p-E2F1 (phospho-Ser337); E2F1 (phospho S337); E2F 1; E2F transcription factor 1; E2F-1; E2f1 E2F transcription factor 1; KIAA4009; mKIAA4009; OTTHUMP00000030661; PBR 3; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP 3; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma associated protein 1; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1; E2F1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-E2F1 (Ser337)
IR:Immunogen Range:L(p-S)PL
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1869
Swiss Prot:Q01094
Source:KLH conjugated Synthesised phosphopeptide derived from human E2F1 around the phosphorylation site of Ser337:L(p-S)PL
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:E2F's are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: Molt-4 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-E2F1 (Ser337) (bs-10012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55-60 kD
Observed band size: 55-60 kD
Sample:
Lane 1: Siha (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Primary:
Anti- phospho-E2F1 (Ser337) (bs-10012R) at 1/1000 dilution
Anti- beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55-60 kD
Observed band size: 55-60 kD
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