Anti-Acetyl Coenzyme A carboJNClase alpha antibody
| 英文名称 | Acetyl Coenzyme A carboJNClase alpha |
| 中文名称 | 乙酰辅酶A羧化酶1/ACCα抗体 |
| 别 名 | ACAC; ACACA; ACACA; ACACA_HUMAN; ACC alpha; ACC-alpha; ACC1; ACC1; ACCA; Acetyl Coenzyme A; Biotin carboJNClase; ACACA_MOUSE; Acetyl Coenzyme A carboJNClase alpha; ACACA_RAT; Acetyl-CoA carboJNClase 1; ACC-alpha. |
DATASHEET
Host:Rabbit
Target Protein:Acetyl Coenzyme A carboJNClase alpha
IR:Immunogen Range:951-1050/2346
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:31
Swiss Prot:Q13085
Source:KLH conjugated synthetic peptide derived from human ACACA:951-1050/2346
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Acetyl-CoA carboJNClase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboJNClation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Acetyl Coenzyme A carboJNClase alpha (bs-11912R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 266 kD
Observed band size: 266 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Acetyl Coenzyme A carboJNClase alpha) Polyclonal Antibody, Unconjugated (bs-11912R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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