Anti-phospho-CXCR4 (Ser339) antibody
| 英文名称 | phospho-CXCR4 (Ser339) |
| 中文名称 | 磷酸化细胞表面趋化因子受体4抗体 |
| 别 名 | p-CXCR4 (phospho S339); p-CD184 (phospho S339); C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; Stromal cell-derived factor 1 receptor; SDF-1 receptor; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; CD184 antigen; CXCR4_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-CXCR4 (Ser339)
IR:Immunogen Range:HS(p-S)VS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:7852
Swiss Prot:P61073
Source:KLH conjugated Synthesised phosphopeptide derived from human CXCR4 around the phosphorylation site of Ser339:HS(p-S)VS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Monomer. Can form dimers.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1μg/Test)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: Uterus (Mouse) Lysate at 40 ug
Lane 4: Lymph node (Mouse) Lysate at 40 ug
Primary:
Anti-phospho-CXCR4 (Ser339) (bs-12256R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Blank control(mouse):Mouse Spleen Cells (fixed with 2% paraformaldehyde for 10 min at 37℃).
Primary Antibody:Rabbit Anti-phospho-CXCR4 (Ser339) antibody (bs-12256R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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