Anti-FLRT2 antibody
| 英文名称 | FLRT2 |
| 中文名称 | 富含亮氨酸跨膜纤连蛋白2抗体 |
| 别 名 | Fibronectin leucine rich transmembrane protein 2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; KIAA0405; Leucine rich repeat transmembrane protein; Leucine rich repeat transmembrane protein FLRT2; FLRT2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:FLRT2
IR:Immunogen Range:201-300/660
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:23768
Swiss Prot:O43155
Source:KLH conjugated synthetic peptide derived from human FLRT2:201-300/660
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:FLRT2 is a 660 amino acid single-pass type I membrane protein that is expressed in pancreas, skeletal muscle, brain and heart. Comprised of one fibronectin type-III domain and ten LRR (leucine-rich) repeats, FLRT2 may play a role in cell adhesion and/or receptor signaling. It is suggested that FLRT2 is involved in mediating events such as NCC (neural crest cell) migration, chondrogenesis and epithelial-mesenchymal interactions during craniofacial development.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FLRT2) Polyclonal Antibody, Unconjugated (bs-12561R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLRT2 Polyclonal Antibody, Unconjugated(bs-12561R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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