Anti-OSR1 + OSR2 antibody (bs-12377R)

Anti-OSR1 + OSR2 antibody

DATASHEET

Host:Rabbit

Target Protein:OSR1 + OSR2

IR:Immunogen Range:181-280/312

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:130497
116039

Swiss Prot:Q8TAX0
Q8N2R0

Source:KLH conjugated synthetic peptide derived from human OSR2 and OSR1:181-280/312 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:OSR2 is a 312 amino acid protein that contains five zinc finger domains. It is expressed in the kidneys, skeletal muscle, testis, and mouse embryos and may be involved in transcriptional activity and osteoblast function. The expression of OSR2 is regulated by C/EBP regulatory elements. OSR2 plays a role in regulating palatal development and expression of alkaline phosphatase. Two isoforms, OSR2A and OSR2B, are produced due to alternative splicing. OSR2B is 36 amino acids shorter than OSR2A and contains only three zinc finger motifs. Both isoforms localize to the nucleus and are thought to exhibit opposite transcriptional activities. Mutations in the gene encoding OSR2 can alter the gene expression of Pax-9 and TGF beta3.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
Danio rerio.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSR1 + OSR2) Polyclonal Antibody, Unconjugated (bs-12377R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Tissue/cell: mouse embryo tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OSR2 Polyclonal Antibody, Unconjugated(bs-12377R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining