Anti-Inversin antibody (bs-12437R)

Anti-Inversin antibody

DATASHEET

Host:Rabbit

Target Protein:Inversin

IR:Immunogen Range:31-130/1065

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:27130

Swiss Prot:Q9Y283

Source:KLH conjugated synthetic peptide derived from human Inversin/Nephrocystin 2:31-130/1065 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Nephrocystin-2 is a 1,065 amino acid protein that exists as three alternatively spliced isoforms and is essential for establishment of the left-right axis and normal renal development. Localizing to the cytoplasm, cytoskeleton, membrane and nucleus, nephrocystin-2 is expressed during presomite-stage embryos and persists in adulthood, with high levels of expression in liver and kidney. Mice expressing nephrocystin-2 mutations are primarily generated by random insertional mutagenesis and result in the reversal of left/right polarity and cyst formation in the kidneys. Furthermore, altered nephrocystin-2 function reverses nodal and lefty expression, indicating that nephrocystin-2 signaling occurs upstream of these proteins involved in the development of asymmetry.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Sheep
Chimpanzee
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
A549(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti- Inversin (bs-12437R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD

Tissue/cell: mouse lymph nodes; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Inversin Polyclonal Antibody, Unconjugated(bs-12437R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining